Mouse Monoclonal Antibody to Phospho Tyrosine
货号:
P15748
别名:
应用:
WB,IP
反应种属:
抗体类型:
Primary antibody
Swissprot:
规格:
目录价
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Specification |
|
---|---|
Host/Isotype | Mouse IgG2a |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
---|---|
WB | 1/500-1/1000 |
IP | 1/100-1/200 |
Product Image
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Lane 1: 15 µg of HeLa lysate Lane 2: 15 µg of sodium vanadate treated HeLa lysate Result: P15748 can detect proteins with phospho tyrosine modification by Western blotting.
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Lane 1: pTyr peptides library; Lane 2: pThr peptides library; Lane 3: pSer peptides library;Lane 4: Tyr peptides library; Lane 5: Thr peptides library; Lane 6: Ser peptides library; Result: P15748 can detect phospho tyrosine peptides by dot blotting;
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Lane 1: HeLa lysate treated with sodium vanadate (1 mM, 45 min) Lane 2: Immunoprecipitated sample from the same lysate as Lane 1 by P15748 Lane 3: The same as Lane 2 but use IgG isotype control antibody Result: P15748 can immunoprecipitate proteins with phospho tyrosine modification.