Rat Monoclonal Antibody to Histone H3K9ac
货号：
P15805
别名：
应用：
WB,IHC,IP
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
P68431/P84243
规格：
目录价
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Specification |
|
|---|---|
| Swissprot | P68431/P84243 |
| Host/Isotype | Rat IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human Histone H3K9ac |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
|---|---|
| WB | 1/500-1/1000 |
| IP | 1/100-1/200 |
| IHC | 1/500 |
Product Image
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Microtiter wells were coated with various peptides. P15805 was used as the primary antibody and peroxidase conjugated goat anti-rat IgG was used as the the secondary antibody. Result: P15805 specifically reacts with acetylated H3K9ac.
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Lane 1: 15 µg of MCF-7 lysate; Lane 2: 15 µg of MCF-7 lysate treated with 1 mM sodium butyrate for 6 hs; Lane 3: 15 µg of MCF-7 lysate treated with 5 mM sodium butyrate for 6 hs;Lane 4: 15 µg of MCF-7 lysate treated with 10 mM sodium butyrate for 6 hs; Result: P15805 can detect H3K9ac by Western blotting.
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Lane 1: 15 µg of HeLa lysate; Lane 2: H3K9ac immunoprecipitated from HeLa lysate by P15805; Lane 3: The same as Lane 2 but use IgG isotype control antibody;Result: P15805 can immunoprecipitate H3K9ac;
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IHC-P analysis in hepatocellular carcinoma tissue by H3K9ac antibody (P15805) Immunohistochemistry was performed using sections of the formalin-fixed paraffin- embedded hepatocellular carcinoma tissue;Result: Vitually all cells are stained at nuclei.
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