Mouse Monoclonal Antibody to HPRT1
货号:
30677
别名:
HPRT; HGPRT
应用:
WB,IHC,FCM
反应种属:
Human
抗体类型:
Primary antibody
Swissprot:
P00492
规格:
目录价
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Description |
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The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout. |
References |
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1. Nucleosides Nucleotides Nucleic Acids. 2011 Dec;30(12):1248-55. 2. Mol Ther. 2010 Jan;18(1):54-62. |
Specification |
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Aliases | HPRT; HGPRT |
Entrez GeneID | 3251 |
Swissprot | P00492 |
clone | 5F11A7 |
WB Predicted band size | 24.6kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Purified recombinant fragment of human HPRT1 (AA: FULL(1-218)) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
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WB | 1/500 - 1/2000 |
IHC | 1/200 - 1/1000 |
FCM | 1/200 - 1/400 |
ELISA | 1/10000 |
Product Image
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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
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Western blot analysis using HPRT1 mouse mAb against Hela (1), A431 (2), A549 (3) cell lysate.
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Flow cytometric analysis of Hela cells using HPRT1 mouse mAb (green) and negative control (red).
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Immunohistochemical analysis of paraffin-embedded human kidney tissues using HPRT1 mouse mAb with DAB staining.