Mouse Monoclonal Antibody to PLCG2
货号：
30725
别名：
FCAS3; APLAID; PLC-IV; PLC-gamma-2
应用：
WB,IF,FCM
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
P16885
规格：
目录价
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Description |
|---|
The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate to 1D-myo-inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG) using calcium as a cofactor. IP3 and DAG are second messenger molecules important for transmitting signals from growth factor receptors and immune system receptors across the cell membrane. Mutations in this gene have been found in autoinflammation, antibody deficiency, and immune dysregulation syndrome and familial cold autoinflammatory syndrome 3. |
References |
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| 1. Mol Cell Biol. 2011 Mar;31(6):1240-51. 2. Am J Hum Genet. 2012 Oct 5;91(4):713-20. |
Specification |
|
|---|---|
| Aliases | FCAS3; APLAID; PLC-IV; PLC-gamma-2 |
| Entrez GeneID | 5336 |
| Swissprot | P16885 |
| clone | 3A8B6 |
| WB Predicted band size | 148kDa |
| Host/Isotype | Mouse IgG2b |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide of human PLCG2 (AA: phospho-Tyrosine 753 of human Phospholipase Cg2(cERDINSLpYDVSRMYV)). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IF | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
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Western blot analysis using PLCG2 mouse mAb against A431 cell lysate.
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Immunofluorescence analysis of Hela cells using PLCG2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
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Flow cytometric analysis of Hela cells using PLCG2 mouse mAb (green) and negative control (red).
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