Mouse Monoclonal Antibody to SLINGSHOT-1L
货号:
30743
别名:
SSH1;SSH1L
应用:
IF,FCM
反应种属:
Human
抗体类型:
Primary antibody
Swissprot:
Q8WYL5
规格:
目录价
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Description |
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The protein encoded by this gene belongs to the slingshot homolog (SSH) family of phosphatases, which regulate actin filament dynamics. The SSH proteins dephosphorylate and activate the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Cofilin is inactivated by kinases such as LIM domain kinase-1 (LIMK1), which may also be dephosphorylated and inactivated by SSH proteins. The SSH family thus appears to play a role in actin dynamics by reactivating cofilin proteins. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. |
References |
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Molecules. 2012 Dec 17;17(12):14975-94. Nan Fang Yi Ke Da Xue Xue Bao. 2011 Jun;31(6):928-32. |
Specification |
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Aliases | SSH1;SSH1L |
Entrez GeneID | 54434 |
Swissprot | Q8WYL5 |
clone | 1A5C8 |
WB Predicted band size | 115.5kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Synthesized peptide fragment of human SLINGSHOT-1L (AA: 1032-1044 ) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
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IF | 1/200 - 1/1000 |
FCM | 1/200 - 1/400 |
ELISA | 1/10000 |
Product Image
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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
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Immunofluorescence analysis of Hela cells using SLINGSHOT-1L mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
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Flow cytometric analysis of Hela cells using SLINGSHOT-1L mouse mAb (green) and negative control (red).