Rat Monoclonal Antibody to PKHD1
货号：
30740
别名：
FPC; Tigm1; AI118496; AI182499
应用：
IHC,IF,FCM
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
E9PZ36
规格：
目录价
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Description |
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Mutations in the PKHD1 gene, which encodes fibrocystin, cause autosomal recessive polycystic kidney disease (ARPKD). Unfortunately, the lack of specific antibodies to the mouse protein impairs the study of splicing, post-translational processing, shedding, and temporal and spatial expression of endogenous fibrocystin at the cellular and subcellular level. |
References |
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| J Am Soc Nephrol. 2011 Dec;22(12):2266-77. Am J Pathol. 2008 Feb;172(2):417-29. |
Specification |
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|---|---|
| Aliases | FPC; Tigm1; AI118496; AI182499 |
| Entrez GeneID | 241035 |
| Swissprot | E9PZ36 |
| clone | 8G12A1 |
| WB Predicted band size | 445kDa |
| Host/Isotype | Rat IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of mouse PKHD1 (AA: 3878-4060) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
|---|---|
| IHC | 1/200 - 1/1000 |
| IF | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
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Immunofluorescence analysis of A431 cells using PKHD1 Rat mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
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Flow cytometric analysis of Hela cells using PKHD1 Rat mAb (green) and negative control (red).
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Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using PKHD1 Rat mAb with DAB staining.
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