Mouse Monoclonal Antibody to MGMT
货号:
30837
别名:
EC 2.1.1.63
应用:
WB,IHC,IF,FCM
反应种属:
Human
抗体类型:
Primary antibody
Swissprot:
P16455
规格:
目录价
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Description |
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MGMT (O-6-methylguanine-DNA methyltransferase) is a protein-coding gene. Diseases associated with MGMT include oligoastrocytoma, and spinal cord astrocytoma. GO annotations related to this gene include DNA-methyltransferase activity and calcium ion binding. |
References |
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1.Neuropathology. 2014 Jun;34(3):268-76. 2.Clin Cancer Res. 2013 Apr 15;19(8):2265-72. |
Specification |
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Aliases | EC 2.1.1.63 |
Entrez GeneID | 4255 |
Swissprot | P16455 |
clone | 1A11F8 |
WB Predicted band size | 21.6kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Purified recombinant fragment of human MGMT (AA: 32-210) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
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WB | 1/500 - 1/2000 |
IHC | 1/200 - 1/1000 |
IF | 1/200 - 1/1000 |
FCM | 1/200 - 1/400 |
ELISA | 1/10000 |
Product Image
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Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);
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Western blot analysis using MGMT mouse mAb against MCF-7 (1), Jurkat (2), HepG2 (3), and PC-3 (4) cell lysate.
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Immunofluorescence analysis of Hela cells using MGMT mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
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Flow cytometric analysis of Hela cells using MGMT mouse mAb (green) and negative control (red).
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Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using MGMT mouse mAb with DAB staining.