Mouse Monoclonal Antibody to Phospho-Histone H2A.X (Ser139)
货号:
P20213
别名:
H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X; gamma H2A.X
应用:
WB,IF
反应种属:
Human,Mouse
抗体类型:
Primary antibody
Swissprot:
P16104
规格:
目录价
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Description |
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Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. |
Specification |
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Aliases | H2A.X; H2AFX; H2a/x; HIST5-2AX; Histone H2A.X; gamma H2A.X |
Entrez GeneID | 3014 |
Swissprot | P16104 |
clone | 9D4 |
WB Predicted band size | Calculated MW: 15 kDa; Observed MW: 15 kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Mouse |
Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X. |
Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
Application |
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WB | 1/500-1/1000 |
IF | 1/50-1/200 |
Product Image
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Western blot analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyureatreated 3T3 lysates using Phospho-Histone H2A.X (Ser139) antibody.
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Immunofluorescence analysis of Phospho-Histone H2A.X (Ser139) in 3T3 or Hydroxyureatreated 3T3 using Phospho-Histone H2A.X (Ser139) antibody.
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Immunofluorescence analysis of Phospho-Histone H2A.X (Ser139) in A549(upper, untreated or Hydroxyureatreated) and Hela(lower, untreated or Hydroxyureatreated) using Phospho-Histone H2A.X (Ser139) antibody.