Rabbit Polyclonal Antibody to Phospho-eIF4E (Ser209)
货号：
P20237
别名：
EIF4E; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein
应用：
WB,IP,IHC,IF
反应种属：
Human,Mouse,Rat
抗体类型：
Primary antibody
Swissprot：
P06730
规格：
目录价
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Description |
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eIF4E, a protein modulates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regulates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. |
Specification |
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|---|---|
| Aliases | EIF4E; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4E; eIF4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein |
| Entrez GeneID | 1977 |
| Swissprot | P06730 |
| WB Predicted band size | Calculated MW: 25 kDa; Observed MW: 25 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Phospho-eIF4E (S209) |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
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|---|---|
| WB | 1/500-1/1000 |
| IP | 1/10-1/20 |
| IHC | 1/50-1/100 |
| IF | 1/50-1/200 |
Product Image
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Western blot analysis of eIF4E (Phospho-S209) in (1) HEK293 lysates; (2) mouse spleen lysates using Phospho-eIF4E (Ser209) antibody.
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Immunohistochemistry analysis of paraffin-embedded mouse kidney using Phospho-eIF4E (S209) antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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