Mouse Monoclonal Antibody to RF1
货号：
30965
别名：
ERF; ETF1; ERF1; TB3-1; D5S1995; SUP45L1
应用：
WB,FCM
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
P62495
规格：
目录价
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Description |
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This gene encodes a class-1 polypeptide chain release factor. The encoded protein plays an essential role in directing termination of mRNA translation from the termination codons UAA, UAG and UGA. This protein is a component of the SURF complex which promotes degradation of prematurely terminated mRNAs via the mechanism of nonsense-mediated mRNA decay (NMD). Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 6, 7, and X. |
References |
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| 1.Nucleic Acids Res. 2013 Apr;41(8):4573-86. 2.Protein Sci. 2012 Jun;21(6):896-903. |
Specification |
|
|---|---|
| Aliases | ERF; ETF1; ERF1; TB3-1; D5S1995; SUP45L1 |
| Entrez GeneID | 2107 |
| Swissprot | P62495 |
| clone | 4F9H12 |
| WB Predicted band size | 49kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human RF1 (AA: 288-437) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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Western blot analysis using mouse mAb against MCF-7 (1), T47D (2), MOLT4 (3), and Raji (4) cell lysate.
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Flow cytometric analysis of Hela cells using RF1 mouse mAb (green) and negative control (red).
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Flow cytometric analysis of HepG2 cells using RF1 mouse mAb (green) and negative control (red).
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