Mouse Monoclonal Antibody to YWHAB
货号:
30990
别名:
HS1; GW128; YWHAA; KCIP-1; HEL-S-1
应用:
WB,IHC,IF,FCM
反应种属:
Human,Mouse,Rat
抗体类型:
Primary antibody
Swissprot:
P31946
规格:
目录价
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Description |
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This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene. |
References |
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1.BMC Res Notes. 2014 Feb 20;7:97. 2.Mol Biol Rep. 2012 Dec;39(12):10647-53. |
Specification |
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Aliases | HS1; GW128; YWHAA; KCIP-1; HEL-S-1 |
Entrez GeneID | 7529 |
Swissprot | P31946 |
clone | 5B5G10 |
WB Predicted band size | 28kDa |
Host/Isotype | Mouse IgG2b |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Mouse,Rat |
Immunogen | Purified recombinant fragment of human YWHAB (AA: 1-246) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
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WB | 1/500 - 1/2000 |
IHC | 1/200 - 1/1000 |
IF | 1/200 - 1/1000 |
FCM | 1/200 - 1/400 |
ELISA | 1/10000 |
Product Image
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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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Western blot analysis using YWHAB mouse mAb against Hela (1), NIH/3T3 (2), C6 (3), A431 (4), K562 (5), PC-12 (6), and U937 (7) cell lysate.
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Immunofluorescence analysis of GC-7901 cells using YWHAB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
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Immunofluorescence analysis of Hela cells using YWHAB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
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Immunofluorescence analysis of HepG2 cells using YWHAB mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
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Flow cytometric analysis of Hela cells using YWHAB mouse mAb (green) and negative control (red).
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Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using YWHAB mouse mAb with DAB staining.
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Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using YWHAB mouse mAb with DAB staining.