Mouse Monoclonal Antibody to BNIP3
货号：
31072
别名：
NIP3
应用：
IHC,IF,FCM
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
Q12983
规格：
目录价
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Description |
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This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation. |
References |
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| 1.Tumour Biol. 2015 Jun;36(6):4731-40. 2.PLoS One. 2014 May 13;9(5):e96733. |
Specification |
|
|---|---|
| Aliases | NIP3 |
| Entrez GeneID | 664 |
| Swissprot | Q12983 |
| clone | 6A5F7 |
| WB Predicted band size | 21.5kDa |
| Host/Isotype | Mouse IgG2a |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human BNIP3 (AA: 50-155) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
|---|---|
| IHC | 1/200 - 1/1000 |
| IF | 1/50 - 1/250 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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Immunofluorescence analysis of Hela cells using BNIP3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
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Flow cytometric analysis of Hela cells using BNIP3 mouse mAb (green) and negative control (red).
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Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using BNIP3 mouse mAb with DAB staining.
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Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using BNIP3 mouse mAb with DAB staining.
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