Rabbit Monoclonal Antibody to SUMO Conjugating Enzyme UBC9
货号:
P22621
别名:
UBE2I; UBC9; UBCE9; SUMO-conjugating enzyme UBC9; SUMO-protein ligase; Ubiquitin carrier protein 9; Ubiquitin carrier protein I; Ubiquitin-conjugating enzyme E2 I; Ubiquitin-protein ligase I; p18
应用:
WB,IP,IHC,IF
反应种属:
Human,Mouse,Rat
抗体类型:
Primary antibody
Swissprot:
P63279
规格:
目录价
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Description |
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The process of SUMO-1 conjugation is similar to that seen with ubiquitin and other forms of post-translational protein modification. Like ubiquitin, SUMO-1 is conjugated to its target protein by the coordinated action of ubiquitin conjugation enzymes E1, E2 and E3. Ubc9 (or ube2M) is a highly conserved, 158 amino acid protein that acts as a SUMO-1 conjugating enzyme. Ubc9 binds to target proteins through their SUMO-1-CS (consensus sequence) domains and interacts with SUMO via the structurally conserved amino-terminal domain. |
Specification |
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Aliases | UBE2I; UBC9; UBCE9; SUMO-conjugating enzyme UBC9; SUMO-protein ligase; Ubiquitin carrier protein 9; Ubiquitin carrier protein I; Ubiquitin-conjugating enzyme E2 I; Ubiquitin-protein ligase I; p18 |
Entrez GeneID | 7329 |
Swissprot | P63279 |
WB Predicted band size | Calculated MW: 18 kDa; Observed MW: 18 kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Mouse,Rat |
Immunogen | A synthetic peptide of human UBE2I/UBC9 |
Formulation | Purified antibody in TBS with 0.05% sodium azide,0.05%BSA and 50% glycerol. |
Application |
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WB | 1/500-1/1000 |
IP | 1/10-1/20 |
IHC | 1/50-1/100 |
IF | 1/50-1/200 |
Product Image
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Western blot analysis of UBE2I/UBC9 in 3T3, PC-12, MEF lysates using UBE2I/UBC9 antibody.
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Western blot analysis of UBE2I/UBC9 in Hela, A549, HL-60 lysates using UBE2I/UBC9 antibody
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Immunocytochemistry analysis of SUMO Conjugating Enzyme UBC9 (green) in Hela using SUMO Conjugating Enzyme UBC9 antibody,and DAPI(blue).
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Immunohistochemistry analysis of paraffin-embedded Human tonsil using UBE2I/UBC9 antibody.High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.