Mouse Monoclonal Antibody to HLA-DRA
货号:
31113
别名:
MLRW; HLA-DRA1
应用:
WB,IF,FCM
反应种属:
Human
抗体类型:
Primary antibody
Swissprot:
P01903
规格:
目录价
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Description |
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HLA-DRA is one of the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha and a beta chain, both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa and its gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. |
References |
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1.Int J Immunogenet. 2014 Dec;41(6):508-11. 2.Am J Rhinol Allergy. 2012 Jan-Feb;26(1):12-7. |
Specification |
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Aliases | MLRW; HLA-DRA1 |
Entrez GeneID | 3122 |
Swissprot | P01903 |
clone | 1C11A5 |
WB Predicted band size | 28.6kDa |
Host/Isotype | Mouse IgG2b |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Purified recombinant fragment of human HLA-DRA (AA: 26-254) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
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WB | 1/500 - 1/2000 |
IF | 1/100 - 1/500 |
FCM | 1/200 - 1/400 |
ELISA | 1/10000 |
Product Image
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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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Western blot analysis using HLA-DRA mouse mAb against Ramos (1), and Raji (2) cell lysate.
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Immunofluorescence analysis of Hela cells using HLA-DRA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
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Flow cytometric analysis of MCF-7 cells using HLA-DRA mouse mAb (green) and negative control (red).