Mouse Monoclonal Antibody to TFAP2A
货号：
31169
别名：
AP-2; BOFS; AP2TF; TFAP2; AP-2alpha
应用：
WB,IF,FCM
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
P05549
规格：
目录价
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Description |
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The protein encoded by this gene is a transcription factor that binds the consensus sequence 5'-GCCNNNGGC-3'. The encoded protein functions as either a homodimer or as a heterodimer with similar family members. This protein activates the transcription of some genes while inhibiting the transcription of others. Defects in this gene are a cause of branchiooculofacial syndrome (BOFS). Three transcript variants encoding different isoforms have been found for this gene. |
References |
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| 1.Int J Clin Exp Pathol. 2014 Nov 26;7(12):8666-74. 2.Hum Pathol. 2012 Nov;43(11):1866-74. |
Specification |
|
|---|---|
| Aliases | AP-2; BOFS; AP2TF; TFAP2; AP-2alpha |
| Entrez GeneID | 7020 |
| Swissprot | P05549 |
| clone | 2H9B5 |
| WB Predicted band size | 48kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Purified recombinant fragment of human TFAP2A (AA: 105-211) expressed in E. Coli. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
|---|---|
| WB | 1/500 - 1/2000 |
| IF | 1/200 - 1/1000 |
| FCM | 1/200 - 1/400 |
| ELISA | 1/10000 |
Product Image
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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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Western blot analysis using TFAP2A mouse mAb against Hela (1), PANC-1 (2), HEK293 (3), and MCF-7 (4) cell lysate.
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Immunofluorescence analysis of Hela cells using TFAP2A mouse mAb. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.
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Immunofluorescence analysis of Hela cells using TFAP2A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
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Flow cytometric analysis of Hela cells using TFAP2A mouse mAb (green) and negative control (red).
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