Rabbit Polyclonal Antibody to NCS1
货号：
P30055
别名：
Neuronal calcium sensor 1, NCS-1, Frequenin homolog, Frequenin-like protein, Frequenin-like ubiquitous protein, NCS1, FLUP, FREQ
应用：
WB,IHC,FCM
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
P62166
规格：
目录价
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Description |
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NCS1 is a member of the neuronal calcium sensor gene family, which encode calcium-binding proteins expressed predominantly in neurons. NCS1 regulates G protein-coupled receptor phosphorylation in a calcium-dependent manner and can substitute for calmodulin. This protein is thought to be associated with secretory granules and may be involved in the regulation of neurosecretion. |
Specification |
|
|---|---|
| Aliases | Neuronal calcium sensor 1, NCS-1, Frequenin homolog, Frequenin-like protein, Frequenin-like ubiquitous protein, NCS1, FLUP, FREQ |
| Entrez GeneID | 23413 |
| Swissprot | P62166 |
| WB Predicted band size | 21.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This NCS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 118-144 amino acids from the Central region of human NCS1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
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|---|---|
| WB | 1/1000 |
| IHC | 1/100-1/500 |
| FCM | 1/10-1/50 |
Product Image
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Western blot analysis of NCS1 Antibody (Center) (Cat.# P30055) in MCF-7 cell line lysates (35ug/lane). NCS1 (arrow) was detected using the purified Pab.
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Flow cytometric analysis of ZR-75-1 cells using NCS1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Formalin-fixed and paraffin-embedded human breast carcinoma reacted with NCS1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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