Rabbit Polyclonal Antibody to EphB6 (N-term S45)
货号：
P30039
别名：
Ephrin type-B receptor 6, HEP, Tyrosine-protein kinase-defective receptor EPH-6, EPHB6
应用：
WB,IHC,FCM
反应种属：
Human, Mouse, Rat
抗体类型：
Primary antibody
Swissprot：
O15197
规格：
目录价
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Description |
|---|
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. EphB6 lacks the kinase activity of most receptor tyrosine kinases and binds to ephrin-B ligands. |
Specification |
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|---|---|
| Aliases | Ephrin type-B receptor 6, HEP, Tyrosine-protein kinase-defective receptor EPH-6, EPHB6 |
| Entrez GeneID | 2051 |
| Swissprot | O15197 |
| WB Predicted band size | 110.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This EphB6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 45-74 amino acids from the N-terminal region of human EphB6. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
Application |
|
|---|---|
| WB | 1/1000-1/2000 |
| IHC | 1/100-1/500 |
| FCM | 1/10-1/50 |
Product Image
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Western blot analysis of anti-EphB6 N-term Pab (Cat. #P30039) in A549 cell lysate. EphB6 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
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Flow cytometric analysis of MCF-7 cells using EphB6 Antibody (N-term S45) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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