Mouse Monoclonal Antibody to ARFGAP1
货号:
31185
别名:
ARF1GAP; HRIHFB2281
应用:
WB,FCM
反应种属:
Human,Mouse
抗体类型:
Primary antibody
Swissprot:
Q8N6T3
规格:
目录价
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Description |
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The protein encoded by this gene is a GTPase-activating protein, which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Alternative splicing results in multiple transcript variants. |
References |
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1.PLoS One. 2014 Nov 14;9(11):e111309. 2.Methods Enzymol. 2001;329:307-16. |
Specification |
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Aliases | ARF1GAP; HRIHFB2281 |
Entrez GeneID | 55738 |
Swissprot | Q8N6T3 |
clone | 1C4E2 |
WB Predicted band size | 44.7kDa |
Host/Isotype | Mouse IgG2b |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Mouse |
Immunogen | Purified recombinant fragment of human ARFGAP1 (AA: 270-414) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
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WB | 1/500 - 1/2000 |
FCM | 1/200 - 1/400 |
ELISA | 1/10000 |
Product Image
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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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Western blot analysis using ARFGAP1 mouse mAb against MOLT4 (1), C2C12 (2), HepG2 (3), MCF-7 (4), and Lncap (5) cell lysate.
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Flow cytometric analysis of HepG2 cells using ARFGAP1 mouse mAb (green) and negative control (red).