Mouse Monoclonal Antibody to APEX1
货号:
31203
别名:
APE; APX; APE1; APEN; APEX; HAP1; REF1
应用:
WB,IHC,FCM
反应种属:
Human,Rat,Monkey
抗体类型:
Primary antibody
Swissprot:
P27695
规格:
目录价
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Description |
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Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. |
References |
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1.Mutat Res Genet Toxicol Environ Mutagen. 2015 Nov;793:19-29. 2.PLoS One. 2015 Dec 1;10(12):e0143289. |
Specification |
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Aliases | APE; APX; APE1; APEN; APEX; HAP1; REF1 |
Entrez GeneID | 328 |
Swissprot | P27695 |
clone | 7A2G7 |
WB Predicted band size | 35.6kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human,Rat,Monkey |
Immunogen | Purified recombinant fragment of human APEX1 (AA: 219-318) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
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WB | 1/500 - 1/2000 |
IHC | 1/200 - 1/1000 |
FCM | 1/200 - 1/400 |
ELISA | 1/10000 |
Product Image
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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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Western blot analysis using APEX1 mouse mAb against Hela (1), Jurkat (2), SW480 (3), A431 (4), HepG2 (5), NIH/3T3 (6), and PC-12 (7) cell lysate.
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Flow cytometric analysis of HeLa cells using APEX1 mouse mAb (green) and negative control (red).
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Flow cytometric analysis of SK-N-SH cells using APEX1 mouse mAb (green) and negative control (red).
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Immunohistochemical analysis of paraffin-embedded human breast cancer tissues using APEX1 mouse mAb with DAB staining.
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Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissues using APEX1 mouse mAb with DAB staining.