Mouse Monoclonal Antibody to CHRNA3
货号:
31232
别名:
LNCR2; PAOD2; NACHRA3
应用:
IF,FCM
反应种属:
Human
抗体类型:
Primary antibody
Swissprot:
P32297
规格:
目录价
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Description |
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This locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described. |
References |
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1.Tumour Biol. 2015 Jul;36(7):4987-92. 2.Int J Mol Sci. 2014 Mar 28;15(4):5446-57. |
Specification |
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Aliases | LNCR2; PAOD2; NACHRA3 |
Entrez GeneID | 1136 |
Swissprot | P32297 |
clone | 6D3A10 |
WB Predicted band size | 57.5kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Purified recombinant fragment of human CHRNA3 (AA: 32-240) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
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IF | 1/200 - 1/1000 |
FCM | 1/200 - 1/400 |
ELISA | 1/10000 |
Product Image
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Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
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Immunofluorescence analysis of Hela cells using CHRNA3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
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Immunofluorescence analysis of SMMC-7721 cells using CHRNA3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
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Flow cytometric analysis of SH-SY5Y cells using CHRNA3 mouse mAb (green) and negative control (red).
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Flow cytometric analysis of SK-N-SH cells using CHRNA3 mouse mAb (green) and negative control (red).