Rabbit Polyclonal Antibody to TSN
货号：
P31074
别名：
Translin, 31--, Component 3 of promoter of RISC, C3PO, TSN
应用：
WB,IHC,IF
反应种属：
Human, Mouse
抗体类型：
Primary antibody
Swissprot：
Q15631
规格：
目录价
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Description |
|---|
This gene encodes a DNA-binding protein which specifically recognizes conserved target sequences at the breakpoint junction of chromosomal translocations. Translin polypeptides form a multimeric structure that is responsible for its DNA-binding activity. Recombination-associated motifs and translin-binding sites are present at recombination hotspots and may serve as indicators of breakpoints in genes which are fused by translocations. These binding activities may play a crucial role in chromosomal translocation in lymphoid neoplasms. |
Specification |
|
|---|---|
| Aliases | Translin, 31--, Component 3 of promoter of RISC, C3PO, TSN |
| Entrez GeneID | 7247 |
| Swissprot | Q15631 |
| WB Predicted band size | 26.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This TSN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-138 amino acids from the Central region of human TSN. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/1000-1/2000 |
| IHC | 1/100-1/500 |
| IF | 1/10-1/50 |
Product Image
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TSN Antibody (Center) (Cat. #P31074) western blot analysis in HL-60,Jurkat,K562 cell line mouse spleen tissue lysates (35ug/lane).This demonstrates the TSN antibody detected the TSN protein (arrow).
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Fluorescent confocal image of Hela cell stained with TSN Antibody(Center)(Cat#P31074).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TSN primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).TSN immunoreactivity is localized to Nucleus and Cytoplasm significantly.
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TSN Antibody (Center) (Cat. #P31074)immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TSN Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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