Mouse Monoclonal Antibody to ALDOA
货号:
31288
别名:
ALDA; GSD12; HEL-S-87p
应用:
IF,FCM
反应种属:
Human
抗体类型:
Primary antibody
Swissprot:
P04075
规格:
目录价
在线咨询
Description |
---|
The protein encoded by this gene, Aldolase A (fructose-bisphosphate aldolase), is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Aldolase A is found in the developing embryo and is produced in even greater amounts in adult muscle. Aldolase A expression is repressed in adult liver, kidney and intestine and similar to aldolase C levels in brain and other nervous tissue. Aldolase A deficiency has been associated with myopathy and hemolytic anemia. Alternative splicing and alternative promoter usage results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 3 and 10. |
References |
---|
1.Cancer Lett. 2016 Apr 28;374(1):127-35. 2.Oncol Rep. 2014 Nov;32(5):2031-7. |
Specification |
|
---|---|
Aliases | ALDA; GSD12; HEL-S-87p |
Entrez GeneID | 226 |
Swissprot | P04075 |
clone | 2H2B1 |
WB Predicted band size | 39.4kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Purified recombinant fragment of human ALDOA (AA: 9-145) expressed in E. Coli. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
---|---|
IF | 1/100 - 1/500 |
FCM | 1/200 - 1/400 |
ELISA | 1/10000 |
Product Image
-
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
-
Immunofluorescence analysis of Hela cells using ALDOA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
-
Flow cytometric analysis of K562 cells using ALDOA mouse mAb (green) and negative control (red).