Rabbit Polyclonal Antibody to GIF
货号：
P31192
别名：
Gastric intrinsic factor, Intrinsic factor, IF, INF, GIF, IFMH
应用：
WB,IHC,IF,FCM
反应种属：
Human, Mouse
抗体类型：
Primary antibody
Swissprot：
P27352
规格：
目录价
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Description |
|---|
This gene is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12. Vitamin B12 is necessary for erythrocyte maturation and mutations in this gene may lead to congenital pernicious anemia. |
Specification |
|
|---|---|
| Aliases | Gastric intrinsic factor, Intrinsic factor, IF, INF, GIF, IFMH |
| Entrez GeneID | 2694 |
| Swissprot | P27352 |
| WB Predicted band size | 45.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This GIF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-218 amino acids from the Central region of human GIF. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/1000 |
| IHC | 1/100-1/500 |
| IF | 1/10-1/50 |
| FCM | 1/10-1/50 |
Product Image
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GIF Antibody (Center) (Cat. #P31192) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the GIF antibody detected the GIF protein (arrow).
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Confocal immunofluorescent analysis of GIF Antibody (Center) (Cat#P31192) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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GIF Antibody (Center) (Cat. #P31192) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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GIF Antibody (Center) (Cat. #P31192)immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GIF Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
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