Mouse Monoclonal Antibody to B7H3
货号:
31371
别名:
CD276; B7-H3; B7RP-2; 4Ig-B7-H3
应用:
IHC,IF,FCM
反应种属:
Human
抗体类型:
Primary antibody
Swissprot:
Q5ZPR3
规格:
目录价
在线咨询
Description |
---|
The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3' UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
References |
---|
1.Int J Clin Exp Pathol. 2015 Nov 1;8(11):13987-95. 2.BMC Cancer. 2014 Aug 20;14:602. |
Specification |
|
---|---|
Aliases | CD276; B7-H3; B7RP-2; 4Ig-B7-H3 |
Entrez GeneID | 80381 |
Swissprot | Q5ZPR3 |
clone | 8C1C2 |
WB Predicted band size | 57.2kDa |
Host/Isotype | Mouse IgG1 |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | Purified recombinant fragment of human B7H3 (AA: extra 29-466) expressed in HEK293 cells. |
Formulation | Purified antibody in PBS with 0.05% sodium azide |
Application |
|
---|---|
IHC | 1/200 - 1/1000 |
IF | 1/100 - 1/500 |
FCM | 1/200 - 1/400 |
ELISA | 1/10000 |
Product Image
-
Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)
-
Immunofluorescence analysis of Hela cells using B7H3 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher (Cat#: 35503)
-
Flow cytometric analysis of Jurkat cells using B7H3 mouse mAb (green) and negative control (red).
-
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using B7H3 mouse mAb with DAB staining.
-
Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissues using B7H3 mouse mAb with DAB staining.