Rabbit Polyclonal Antibody to POLR2C
货号：
P32542
别名：
DNA-directed RNA polymerase II subunit RPB3, RNA polymerase II subunit 3, RNA polymerase II subunit B3, DNA-directed RNA polymerase II 33 kDa polypeptide, RPB33, DNA-directed RNA polymerase II subunit C, RPB31, POLR2C
应用：
WB,IHC,IF
反应种属：
Human, Mouse
抗体类型：
Primary antibody
Swissprot：
P19387
规格：
目录价
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Description |
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DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB3 is part of the core element with the central large cleft and the clamp element that moves to open and close the cleft (By similarity). |
Specification |
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|---|---|
| Aliases | DNA-directed RNA polymerase II subunit RPB3, RNA polymerase II subunit 3, RNA polymerase II subunit B3, DNA-directed RNA polymerase II 33 kDa polypeptide, RPB33, DNA-directed RNA polymerase II subunit C, RPB31, POLR2C |
| Entrez GeneID | 5432 |
| Swissprot | P19387 |
| WB Predicted band size | 31.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This POLR2C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 200-228 amino acids from the C-terminal region of human POLR2C. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/1000 |
| IHC | 1/100 |
| IF | 1/25 |
Product Image
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POLR2C Antibody (C-term) (Cat. #P32542) western blot analysis in Hela,U251 cell line and mouse skeletal muscle tissue lysates (35ug/lane).This demonstrates the POLR2C antibody detected the POLR2C protein (arrow).
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Fluorescent image of Hela cells stained with POLR2C Antibody (C-term)(Cat#P32542). P32542 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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Formalin-fixed and paraffin-embedded H.brain, H.cerebellum tissue reacted with POLR2C Antibody (C-term) (Cat#P32542).
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