Rabbit Polyclonal Antibody to ADH7
货号：
P32618
别名：
Alcohol dehydrogenase class 4 mu/sigma chain, Alcohol dehydrogenase class IV mu/sigma chain, Gastric alcohol dehydrogenase, Retinol dehydrogenase, ADH7
应用：
WB,IHC,IF,FCM
反应种属：
Human, Mouse, Rat
抗体类型：
Primary antibody
Swissprot：
P40394
规格：
目录价
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Description |
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This gene encodes class IV alcohol dehydrogenase 7 mu or sigma subunit, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The enzyme encoded by this gene is inefficient in ethanol oxidation, but is the most active as a retinol dehydrogenase; thus it may participate in the synthesis of retinoic acid, a hormone important for cellular differentiation. The expression of this gene is much more abundant in stomach than liver, thus differing from the other known gene family members. |
Specification |
|
|---|---|
| Aliases | Alcohol dehydrogenase class 4 mu/sigma chain, Alcohol dehydrogenase class IV mu/sigma chain, Gastric alcohol dehydrogenase, Retinol dehydrogenase, ADH7 |
| Entrez GeneID | 131 |
| Swissprot | P40394 |
| WB Predicted band size | 41.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This ADH7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 318-346 amino acids from the C-terminal region of human ADH7. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/1000 |
| IHC | 1/100-1/500 |
| IF | 1/10-1/50 |
| FCM | 1/10-1/50 |
Product Image
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Western blot analysis of lysates from HepG2, SW480 cell line (from left to right), using ADH7 Antibody (C-Term)(Cat. #P32618). P32618 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Confocal immunofluorescent analysis of ADH7 Antibody (C-Term) (Cat. #P32618) with NCI-H460 cell followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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ADH7 Antibody (C-Term) (Cat. #P32618) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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ADH7 Antibody (C-Term) (Cat. #P32618) IHC analysis in formalin fixed and paraffin embedded lung tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ADH7 Antibody (C-Term) for immunohistochemistry. Clinical relevance has not been evaluated.
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