Rabbit Polyclonal Antibody to DFFB
货号:
P32643
别名:
DNA fragmentation factor subunit beta, 3---, Caspase-activated deoxyribonuclease, CAD, Caspase-activated DNase, Caspase-activated nuclease, CPAN, DNA fragmentation factor 40 kDa subunit, DFF-40, DFFB, CAD, DFF2, DFF40
应用:
WB,IHC,FCM
反应种属:
Human, Mouse, Rat
抗体类型:
Primary antibody
Swissprot:
O76075
规格:
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Description |
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Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. |
Specification |
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Aliases | DNA fragmentation factor subunit beta, 3---, Caspase-activated deoxyribonuclease, CAD, Caspase-activated DNase, Caspase-activated nuclease, CPAN, DNA fragmentation factor 40 kDa subunit, DFF-40, DFFB, CAD, DFF2, DFF40 |
Entrez GeneID | 1677 |
Swissprot | O76075 |
WB Predicted band size | 39.1kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse, Rat |
Immunogen | This DFFB antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 203-238 amino acids from the Central region of human DFFB. |
Application |
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WB | 1/1000 |
IHC | 1/100-1/500 |
FCM | 1/25 |
Product Image
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Western blot analysis of lysate from A431 cell line, using DFFB Antibody (Center)(Cat. #P32643). P32643 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug.
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Flow cytometric analysis of Hela cells using DFFB Antibody (Center)(green, Cat#P32643) compared to an isotype control of rabbit IgG(blue). P32643 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody.
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Immunohistochemical analysis of paraffin-embedded M.kidney section using DFFB Antibody (Center)(Cat#P32643). P32643 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Immunohistochemical analysis of paraffin-embedded H.kidney section using DFFB Antibody (Center)(Cat#P32643). P32643 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.