Rabbit Polyclonal Antibody to PEMT (N-term)
货号：
P32704
别名：
Phosphatidylethanolamine N-methyltransferase, PEAMT, PEMT, PEMT2, PEMT, PEMPT, PNMT
应用：
WB,IHC
反应种属：
Human, Mouse, Rat
抗体类型：
Primary antibody
Swissprot：
Q9UBM1
规格：
目录价
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Description |
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This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. The protein localizes to the endoplasmic reticulum and mitochondria-associated membranes. The gene is within the Smith-Magenis syndrome region on chromosome 17. Alternate splicing of this gene results in three transcript variants encoding two different isoforms. |
Specification |
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|---|---|
| Aliases | Phosphatidylethanolamine N-methyltransferase, PEAMT, PEMT, PEMT2, PEMT, PEMPT, PNMT |
| Entrez GeneID | 10400 |
| Swissprot | Q9UBM1 |
| WB Predicted band size | 22.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This PEMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PEMT. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
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|---|---|
| WB | 1/1000 |
| IHC | 1/100-1/500 |
Product Image
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Western blot analysis of lysates from CEM, rat L6 cell line and human liver tissue lysate(from left to right), using PEMT Antibody (N-term)(Cat. #P32704). P32704 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Western blot analysis of anti-PEMT Antibody (N-term) Pab (Cat.#P32704) in mouse kidney tissue lysates (35ug/lane). PEMT(arrow) was detected using the purified Pab.
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Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PEMT antibody (N-term) (Cat.#P32704), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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