Rabbit Polyclonal Antibody to XAF1
货号：
P32668
别名：
XIAP-associated factor 1, BIRC4-binding protein, XAF1, BIRC4BP, XIAPAF1
应用：
WB,IF
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
Q6GPH4
规格：
目录价
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Description |
|---|
Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti- caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha- induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondra, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells. |
Specification |
|
|---|---|
| Aliases | XIAP-associated factor 1, BIRC4-binding protein, XAF1, BIRC4BP, XIAPAF1 |
| Entrez GeneID | 54739 |
| Swissprot | Q6GPH4 |
| WB Predicted band size | 34.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This XAF1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 235-269 amino acids from the C-terminal region of human XAF1. |
Application |
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|---|---|
| WB | 1/1000 |
| IF | 1/25 |
Product Image
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Western blot analysis of lysates from human liver and spleen tissue lysate(from left to right), using XAF1 Antibody (C-term)(Cat. #P32668). P32668 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
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Fluorescent image of Hela cells stained with XAF1 Antibody (C-term)(Cat#P32668). P32668 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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