Rabbit Polyclonal Antibody to (Mouse) Eras (N-term)
货号：
P32939
别名：
GTPase ERas, E-Ras, Embryonic stem cell-expressed Ras, Eras
应用：
WB,FCM
反应种属：
Mouse
抗体类型：
Primary antibody
Swissprot：
Q7TN89
规格：
目录价
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Description |
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Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the tumor-like growth properties of embryonic stem cells. |
Specification |
|
|---|---|
| Aliases | GTPase ERas, E-Ras, Embryonic stem cell-expressed Ras, Eras |
| Entrez GeneID | 353283 |
| Swissprot | Q7TN89 |
| WB Predicted band size | 24.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This Mouse Eras antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 19-52 amino acids from the N-terminal region of mouse Eras. |
Application |
|
|---|---|
| WB | 1/1000 |
| FCM | 1/25 |
Product Image
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Western blot analysis of lysate from mouse F9 cell line, using Eras Antibody (N-term)(Cat. #P32939). P32939 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
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Overlay histogram showing F9 cells stained with P32939 (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P32939, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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