Rabbit Polyclonal Antibody to (Mouse) Melk
货号：
P33027
别名：
Maternal embryonic leucine zipper kinase, Protein kinase PK38, mPK38, Tyrosine-protein kinase MELK, Melk, Kiaa0175, Pk38
应用：
WB,FCM
反应种属：
Mouse
抗体类型：
Primary antibody
Swissprot：
Q61846
规格：
目录价
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Description |
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Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Has a broad substrate specificity; phosphorylates BCL2L14, CDC25B, MAP3K5/ASK1 and ZNF622. Acts as an activator of apoptosis by phosphorylating and activating MAP3K5/ASK1. Acts as a regulator of cell cycle, notably by mediating phosphorylation of CDC25B, promoting localization of CDC25B to the centrosome and the spindle poles during mitosis. Plays a key role in cell proliferation. Required for proliferation of embryonic and postnatal multipotent neural progenitors. Phosphorylates and inhibits BCL2L14. Also involved in the inhibition of spliceosome assembly during mitosis by phosphorylating ZNF622, thereby contributing to its redirection to the nucleus. May also play a role in primitive hematopoiesis. |
Specification |
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|---|---|
| Aliases | Maternal embryonic leucine zipper kinase, Protein kinase PK38, mPK38, Tyrosine-protein kinase MELK, Melk, Kiaa0175, Pk38 |
| Entrez GeneID | 17279 |
| Swissprot | Q61846 |
| WB Predicted band size | 72.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Mouse |
| Immunogen | This mouse Melk antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 434-468 amino acids from the C-terminal region of mouse Melk. |
Application |
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|---|---|
| WB | 1/1000 |
| FCM | 1/25 |
Product Image
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Anti-Melk Antibody (C-term) at 1:1000 dilution + mouse testis lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 73 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing MCF-7 cells stained with P33027 (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33027, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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