Rabbit Polyclonal Antibody to Bcl-G BH3 Domain
货号：
P33041
别名：
Apoptosis facilitator Bcl-2-like protein 14, Bcl2-L-14, Apoptosis regulator Bcl-G, BCL2L14, BCLG
应用：
WB,IHC
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
Q9BZR8
规格：
目录价
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Description |
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Bcl-G belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. Overexpression of Bcl-G has been shown to induce apoptosis in cells. |
Specification |
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|---|---|
| Aliases | Apoptosis facilitator Bcl-2-like protein 14, Bcl2-L-14, Apoptosis regulator Bcl-G, BCL2L14, BCLG |
| Entrez GeneID | 79370 |
| Swissprot | Q9BZR8 |
| WB Predicted band size | 36.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This Bcl antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 194-229 amino acids from human Bcl. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
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|---|---|
| WB | 1/1000-1/2000 |
| IHC | 1/100-1/500 |
Product Image
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Anti-Bcl-G-BH3 Antibodyat 1:2000 dilution + human lung lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 37 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Western blot analysis of anti-Bcl-G BH3 Domain Pab (Cat. #P33041) in K562 cell line lysates (35ug/lane). Bcl-G BH3 Domain (arrow) was detected using the purified Pab.
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Cell lysates were subjected to Western blotting to determine the expression of Bcl-gS protein. The levels of alpha-tubulin were determined to assure equal loading.
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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