Rabbit Polyclonal Antibody to (Mouse) Smad1
货号：
P33023
别名：
Mothers against decapentaplegic homolog 1, MAD homolog 1, Mothers against DPP homolog 1, Dwarfin-A, Dwf-A, Mothers-against-DPP-related 1, Mad-related protein 1, mMad1, SMAD family member 1, SMAD 1, Smad1, Smad1, Madh1, Madr1
应用：
WB,IHC
反应种属：
Human, Mouse, Rat
抗体类型：
Primary antibody
Swissprot：
P70340
规格：
目录价
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Description |
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Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD) (By similarity). May play a role in the initiation and maintenance of spermatogenesis. SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1 (By similarity). May act synergistically with SMAD4 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene [removed]PubMed:15329343). |
Specification |
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| Aliases | Mothers against decapentaplegic homolog 1, MAD homolog 1, Mothers against DPP homolog 1, Dwarfin-A, Dwf-A, Mothers-against-DPP-related 1, Mad-related protein 1, mMad1, SMAD family member 1, SMAD 1, Smad1, Smad1, Madh1, Madr1 |
| Entrez GeneID | 17125 |
| Swissprot | P70340 |
| WB Predicted band size | 52.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This Mouse Smad1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 154-187 amino acids from the Central region of Mouse Smad1. |
Application |
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| WB | 1/1000 |
| IHC | 1/100-1/500 |
Product Image
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Anti-Smad1 Antibody (Center) at 1:1000 dilution + mouse skeletal muscle lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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P33023 staining (Mouse) Smad1 in Mouse heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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