Rabbit Polyclonal Antibody to EGF
货号：
P33039
别名：
Pro-epidermal growth factor, EGF, Epidermal growth factor, Urogastrone, EGF
应用：
WB,IHC
反应种属：
Human, Mouse
抗体类型：
Primary antibody
Swissprot：
P01133
规格：
目录价
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Description |
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This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants. |
Specification |
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|---|---|
| Aliases | Pro-epidermal growth factor, EGF, Epidermal growth factor, Urogastrone, EGF |
| Entrez GeneID | 1950 |
| Swissprot | P01133 |
| WB Predicted band size | 134.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This EGF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 690-720 amino acids from the Central region of human EGF. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
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|---|---|
| WB | 1/1000 |
| IHC | 1/100-1/500 |
Product Image
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All lanes : Anti-EGF Antibody (Center) at 1:2000 dilution Lane 1: M.stomach tissue lysates Lane 2: A549 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 134 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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P33039 staining EGF in Human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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P33039 staining EGF in Human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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