Rabbit Polyclonal Antibody to PROX-1-S514
货号：
P33047
别名：
Prospero homeobox protein 1, Homeobox prospero-like protein PROX1, PROX-1, PROX1
应用：
WB,FCM
反应种属：
Human, Mouse
抗体类型：
Primary antibody
Swissprot：
Q92786
规格：
目录价
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Description |
|---|
Apolipoprotein H has been implicated in a variety of physiologic pathways including lipoprotein metabolism, coagulation, and the production of antiphospholipid autoantibodies. APOH may be a required cofactor for anionic phospholipid binding by the antiphospholipid autoantibodies found in sera of many patients with lupus and primary antiphospholipid syndrome, but it does not seem to be required for the reactivity of antiphospholipid autoantibodies associated with infections. |
Specification |
|
|---|---|
| Aliases | Prospero homeobox protein 1, Homeobox prospero-like protein PROX1, PROX-1, PROX1 |
| Entrez GeneID | 5629 |
| Swissprot | Q92786 |
| WB Predicted band size | 83.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This PROX1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 492-520 amino acids from human PROX1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/1000 |
| FCM | 1/25 |
Product Image
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All lanes : Anti-PROX-1-S514 Antibody at 1:1000 dilution Lane 1: HepG2 whole cell lysates Lane 2: SH-SY5Y whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 83 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Overlay histogram showing A549 cells stained with P33047 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33047, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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