Rabbit Polyclonal Antibody to ATP2B3
货号：
P33061
别名：
应用：
WB,IF
反应种属：
Human, Rat
抗体类型：
Primary antibody
Swissprot：
Q16720
规格：
目录价
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Specification |
|
|---|---|
| Entrez GeneID | 492 |
| Swissprot | Q16720 |
| WB Predicted band size | 134.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | This ATP2B3 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1161-1194 amino acids from the C-terminal region of human ATP2B3. |
Application |
|
|---|---|
| WB | 1/2000 |
| IF | 1/25 |
Product Image
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Anti-ATP2B3 Antibody (C-term)at 1:2000 dilution + U-87 MG whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 134 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Hela (Human Cervical epithelial adenocarcinoma cell line) cells labeling ATP2B3 with P33061 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing membrane staining on Hela cell line.The nuclear counter stain is DAPI (blue).
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