Rabbit Polyclonal Antibody to CBFA2T2 (N-term)
货号：
P33044
别名：
Protein CBFA2T2, ETO homologous on chromosome 20, MTG8-like protein, MTG8-related protein 1, Myeloid translocation-related protein 1, p85, CBFA2T2, EHT, MTGR1
应用：
WB,IF
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
O43439
规格：
目录价
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Description |
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In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several transcript variants are thought to exist for this gene, but the full-length natures of only three have been described. |
Specification |
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|---|---|
| Aliases | Protein CBFA2T2, ETO homologous on chromosome 20, MTG8-like protein, MTG8-related protein 1, Myeloid translocation-related protein 1, p85, CBFA2T2, EHT, MTGR1 |
| Entrez GeneID | 9139 |
| Swissprot | O43439 |
| WB Predicted band size | 67.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This CBFA2T2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-29 amino acids from the N-terminal region of human CBFA2T2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
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|---|---|
| WB | 1/1000 |
| IF | 1/10-1/50 |
Product Image
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All lanes : Anti-CBFA2T2 Antibody (N-term) at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: Jurkat whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 67 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Fluorescent image of Hela cell stained with CBFA2T2 Antibody (N-term)(Cat#P33044).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CBFA2T2 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃).CBFA2T2 immunoreactivity is localized to Nucleus significantly.
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