Rabbit Polyclonal Antibody to Dnmt3b
货号：
P33133
别名：
DNA (cytosine-5)-methyltransferase 3B, Dnmt3b, DNA methyltransferase HsaIIIB, DNA MTase HsaIIIB, MHsaIIIB, DNMT3B
应用：
WB,IHC
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
Q9UBC3
规格：
目录价
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Description |
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CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. Dnmt3b is a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF)syndrome. |
Specification |
|
|---|---|
| Aliases | DNA (cytosine-5)-methyltransferase 3B, Dnmt3b, DNA methyltransferase HsaIIIB, DNA MTase HsaIIIB, MHsaIIIB, DNMT3B |
| Entrez GeneID | 1789 |
| Swissprot | Q9UBC3 |
| WB Predicted band size | 95.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This Dnmt3b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-417 amino acids from human Dnmt3b. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
Application |
|
|---|---|
| WB | 1/1000-1/2000 |
| IHC | 1/100-1/500 |
Product Image
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Anti-Dnmt3b Antibody (C403)at 1:2000 dilution + T47D whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 95 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Western blot analysis of anti-Dnmt3b Pab (Cat. #P33133) in T47-D cell lysate. Dnmt3b (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
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Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with Dnmt3b antibody (Cat.#P33133), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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