Rabbit Polyclonal Antibody to ITGA7 (N-term)
货号：
P33153
别名：
Integrin alpha-7, Integrin alpha-7 heavy chain, Integrin alpha-7 light chain, Integrin alpha-7 70 kDa form, ITGA7
应用：
WB,IHC,IF,FCM
反应种属：
Human, Mouse, Rat
抗体类型：
Primary antibody
Swissprot：
Q13683
规格：
目录价
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Description |
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Integrin alpha-7/beta-1 is the primary laminin receptor on skeletal myoblasts and adult myofibers. During myogenic differentiation, it may induce changes in the shape and mobility of myoblasts, and facilitate their localization at laminin-rich sites of secondary fiber formation. It is involved in the maintenance of the myofibers cytoarchitecture as well as for their anchorage, viability and functional integrity. Isoform Alpha-7X2B and isoform Alpha-7X1B promote myoblast migration on laminin 1 and laminin 2/4, but isoform Alpha-7X1B is less active on laminin 1 (In vitro). Acts as Schwann cell receptor for laminin-2. Acts as a receptor of COMP and mediates its effect on vascular smooth muscle cells (VSMCs) maturation (By similarity). Required to promote contractile phenotype acquisition in differentiated airway smooth muscle (ASM) cells. |
Specification |
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|---|---|
| Aliases | Integrin alpha-7, Integrin alpha-7 heavy chain, Integrin alpha-7 light chain, Integrin alpha-7 70 kDa form, ITGA7 |
| Entrez GeneID | 3679 |
| Swissprot | Q13683 |
| WB Predicted band size | 128.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This ITGA7 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 247-279 amino acids from the N-terminal region of human ITGA7. |
Application |
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|---|---|
| WB | 1/2000 |
| IHC | 1/100-1/500 |
| IF | 1/25 |
| FCM | 1/25 |
Product Image
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All lanes : Anti-ITGA7 Antibody (N-term) at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: A431 whole cell lysates Lane 3: Jurkat whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 129 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling ITGA7 with P33153 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing vesicles,cytoplasm and weakly nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
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Overlay histogram showing U-2 OS cells stained with P33153 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33153, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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P33153 staining ITGA7 in human skeletal muscle sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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