Rabbit Polyclonal Antibody to BCL9 (N-Term)
货号：
P33232
别名：
B-cell CLL/lymphoma 9 protein, B-cell lymphoma 9 protein, Bcl-9, Protein legless homolog, BCL9
应用：
WB,IF
反应种属：
Human, Mouse
抗体类型：
Primary antibody
Swissprot：
O00512
规格：
目录价
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Description |
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Involved in signal transduction through the Wnt pathway. Promotes beta-catenin's transcriptional activity (By similarity). |
Specification |
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| Aliases | B-cell CLL/lymphoma 9 protein, B-cell lymphoma 9 protein, Bcl-9, Protein legless homolog, BCL9 |
| Entrez GeneID | 607 |
| Swissprot | O00512 |
| WB Predicted band size | 149.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This BCL9 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 272-304 amino acids from human BCL9. |
Application |
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|---|---|
| WB | 1/2000 |
| IF | 1/25 |
Product Image
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All lanes : Anti-BCL9 Antibody (N-Term) at 1:2000 dilution Lane 1: mouse spleen lysates Lane 2: human testis lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 149 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling BCL9 with P33232 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).
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