Mouse Monoclonal Antibody to NMI
货号：
P33459
别名：
N-myc-interactor, Nmi, N-myc and STAT interactor, NMI
应用：
WB,IF,FCM
反应种属：
Human, Mouse
抗体类型：
Primary antibody
Swissprot：
Q13287
规格：
目录价
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Description |
|---|
May be involved in augmenting coactivator protein recruitment to a group of sequence-specific transcription factors. Augments cytokine-mediated STAT transcription. Enhances CBP/p300 coactivator protein recruitment to STAT1 and STAT5. |
Specification |
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|---|---|
| Aliases | N-myc-interactor, Nmi, N-myc and STAT interactor, NMI |
| Entrez GeneID | 9111 |
| Swissprot | Q13287 |
| WB Predicted band size | 35.1kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This NMI antibody is generated from a mouse immunized with a recombinant protein of human NMI. |
Application |
|
|---|---|
| WB | 1/2000 |
| IF | 1/25 |
| FCM | 1/25 |
Product Image
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Anti-NMI Antibody at 1:2000 dilution + A431 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 35 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling NMI with P33459 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and nucleus staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
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Overlay histogram showing K562 cells stained with P33459(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33459, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OJ192088) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was mouse IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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