Rabbit Polyclonal Antibody to CD3Z (N-term)
货号：
P33531
别名：
T-cell surface glycoprotein CD3 zeta chain, T-cell receptor T3 zeta chain, CD247, CD247, CD3Z, T3Z, TCRZ
应用：
WB,FCM
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
P20963
规格：
目录价
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Description |
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T-cell receptor zeta, together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. |
Specification |
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|---|---|
| Aliases | T-cell surface glycoprotein CD3 zeta chain, T-cell receptor T3 zeta chain, CD247, CD247, CD3Z, T3Z, TCRZ |
| Entrez GeneID | 919 |
| Swissprot | P20963 |
| WB Predicted band size | 18.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This CD3Z antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-31 amino acids from the N-terminal region of human CD3Z. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
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|---|---|
| WB | 1/1000 |
| FCM | 1/10-1/50 |
Product Image
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All lanes : Anti-CD3Z Antibody (N-term) at 1:1000 dilution Lane 1: Jurkat whole cell lysate Lane 2: MOLT-4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 19 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Western blot analysis of anti-CD3Z Antibody (N-term) (RB13817) in Ramos cell line lysates (35ug/lane). CD3Z(arrow) was detected using the purified Pab.
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Flow cytometric analysis of Ramos cells using CD3Z Antibody (N-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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