Rabbit Polyclonal Antibody to LYN (N-term)
货号：
P33579
别名：
Tyrosine-protein kinase Lyn, Lck/Yes-related novel protein tyrosine kinase, V-yes-1 Yamaguchi sarcoma viral related oncogene homolog, p53Lyn, p56Lyn, LYN, JTK8
应用：
WB,IHC,FCM
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
P07948
规格：
目录价
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Description |
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Lyn is a protein tyrosine kinase which is important in cellular signaling. It contains an SH2 and SH3 domain and has been found to be cleaved after activation of caspases in apoptosis. |
Specification |
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| Aliases | Tyrosine-protein kinase Lyn, Lck/Yes-related novel protein tyrosine kinase, V-yes-1 Yamaguchi sarcoma viral related oncogene homolog, p53Lyn, p56Lyn, LYN, JTK8 |
| Entrez GeneID | 4067 |
| Swissprot | P07948 |
| WB Predicted band size | 58.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This LYN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 32-62 amino acids from the N-terminal region of human LYN. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
Application |
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|---|---|
| WB | 1/1000-1/2000 |
| IHC | 1/100-1/500 |
| FCM | 1/10-1/50 |
Product Image
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Anti-LYN Antibody (E46) at 1:1000 dilution + Raji whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 59 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Western blot analysis of anti-LYN Antibody (N-term) (Cat.#P33579) in HL60 cell line lysates (35ug/lane). LYN (arrow) was detected using the purified Pab.
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LYN Antibody (N-term) (Cat. #P33579) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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