Rabbit Polyclonal Antibody to ARV1 (N-term)
货号：
P33593
别名：
Protein ARV1, hARV1, ARV1
应用：
WB,IHC,FCM
反应种属：
Human, Mouse, Rat
抗体类型：
Primary antibody
Swissprot：
Q9H2C2
规格：
目录价
在线咨询
Description |
|---|
May act as a mediator of sterol homeostasis (Potential). |
Specification |
|
|---|---|
| Aliases | Protein ARV1, hARV1, ARV1 |
| Entrez GeneID | 64801 |
| Swissprot | Q9H2C2 |
| WB Predicted band size | 31.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This ARV1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-51 amino acids from the N-terminal region of human ARV1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/1000-1/2000 |
| IHC | 1/100-1/500 |
| FCM | 1/25 |
Product Image
-
All lanes : Anti-ARV1 Antibody (N-term) at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: human kidney lysate Lane 3: HL-60 whole cell lysate Lane 4: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
-
ARV1 Antibody (N-term) (Cat. #P33593) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the ARV1 antibody detected the ARV1 protein (arrow).
-
ARV1 Antibody (N-term) (Cat. #P33593) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the ARV1 antibody detected the ARV1 protein (arrow).
-
Overlay histogram showing A2058 cells stained with P33593(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33593, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
-
ARV1 antibody (N-term) (Cat. #P33593) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ARV1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
鄂公网安备42018502007531号
