Rabbit Polyclonal Antibody to ZWINT
货号：
P33652
别名：
ZW10 interactor, ZW10-interacting protein 1, Zwint-1, ZWINT
应用：
WB,IHC,FCM
反应种属：
Human, Mouse
抗体类型：
Primary antibody
Swissprot：
O95229
规格：
目录价
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Description |
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ZWINT is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. |
Specification |
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|---|---|
| Aliases | ZW10 interactor, ZW10-interacting protein 1, Zwint-1, ZWINT |
| Entrez GeneID | 11130 |
| Swissprot | O95229 |
| WB Predicted band size | 31.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This ZWINT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 59-88 amino acids from the Central region of human ZWINT. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
Application |
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|---|---|
| WB | 1/1000 |
| IHC | 1/100-1/500 |
| FCM | 1/10-1/50 |
Product Image
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Anti-ZWINT Antibody (Center) at 1:1000 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 31 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Western blot analysis of ZWINT antibody (Center) (Cat. #P33652) in mouse cerebellum tissue lysates (35ug/lane). ZWINT (arrow) was detected using the purified Pab.
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Flow cytometric analysis of widr cells using ZWINT Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Formalin-fixed and paraffin-embedded human brain tissue reacted with ZWINT Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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