Rabbit Polyclonal Antibody to NSUN2
货号：
P33698
别名：
tRNA (cytosine(34)-C(5))-methyltransferase, Myc-induced SUN domain-containing protein, Misu, NOL1/NOP2/Sun domain family member 2, Substrate of AIM1/Aurora kinase B, tRNA (cytosine-5-)-methyltransferase, tRNA methyltransferase 4 homolog, hTrm4, NSUN2, SAK
应用：
WB
反应种属：
Human, Mouse
抗体类型：
Primary antibody
Swissprot：
Q08J23
规格：
目录价
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Description |
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Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu(CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu(CAA) (Brzezicha et al., 2006 [PubMed 17071714]). |
Specification |
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| Aliases | tRNA (cytosine(34)-C(5))-methyltransferase, Myc-induced SUN domain-containing protein, Misu, NOL1/NOP2/Sun domain family member 2, Substrate of AIM1/Aurora kinase B, tRNA (cytosine-5-)-methyltransferase, tRNA methyltransferase 4 homolog, hTrm4, NSUN2, SAKI, TRM4 |
| Entrez GeneID | 54888 |
| Swissprot | Q08J23 |
| WB Predicted band size | 86.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This NSUN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 423-451 amino acids from the Central region of human NSUN2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
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|---|---|
| WB | 1/1000 |
Product Image
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All lanes : Anti-NSUN2 Antibody (Center) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 86 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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NSUN2 Antibody (Center) (Cat. #P33698) western blot analysis in mouse spleen tissue lysates (35ug/lane).This demonstrates the NSUN2 antibody detected the NSUN2 protein (arrow).
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