Rabbit Polyclonal Antibody to CAF-1 (N-term)
货号：
P33706
别名：
CCR4-NOT transcription complex subunit 7, BTG1-binding factor 1, CCR4-associated factor 1, CAF-1, Caf1a, CNOT7, CAF1
应用：
WB,IHC,IF
反应种属：
Human
抗体类型：
Primary antibody
Swissprot：
Q9UIV1
规格：
目录价
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Description |
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The protein encoded by this gene binds to an anti-proliferative protein, B-cell translocation protein 1, which negatively regulates cell proliferation. Binding of the two proteins, which is driven by phosphorylation of the anti-proliferative protein, causes signaling events in cell division that lead to changes in cell proliferation associated with cell-cell contact. The protein has both mouse and yeast orthologs. Alternate splicing of this gene results in two transcript variants encoding different isoforms. |
Specification |
|
|---|---|
| Aliases | CCR4-NOT transcription complex subunit 7, BTG1-binding factor 1, CCR4-associated factor 1, CAF-1, Caf1a, CNOT7, CAF1 |
| Entrez GeneID | 29883 |
| Swissprot | Q9UIV1 |
| WB Predicted band size | 32.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This CAF-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 34-61 amino acids from the N-terminal region of human CAF-1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/1000 |
| IHC | 1/100-1/500 |
| IF | 1/10-1/50 |
Product Image
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Anti-CAF-1 Antibody (N-term) at 1:1000 dilution + HUVEC whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 33 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Fluorescent confocal image of Hela cell stained with CAF-1 Antibody (N-term)(Cat#AP11112a).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CAF-1 primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). CAF-1 immunoreactivity is localized to Cytoplasm and Nucleus significantly.
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CAF-1 Antibody (N-term) (Cat. #P33706)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of CAF-1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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