Rabbit Polyclonal Antibody to MYBPC3 (N-term)
货号：
P33721
别名：
Myosin-binding protein C, cardiac-type, Cardiac MyBP-C, C-protein, cardiac muscle isoform, MYBPC3
应用：
WB,IHC
反应种属：
Human, Mouse, Rat
抗体类型：
Primary antibody
Swissprot：
Q14896
规格：
目录价
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Description |
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MYBPC3 encodes the cardiac isoform of myosin-binding protein C. Myosin-binding protein C is a myosin-associated protein found in the cross-bridge-bearing zone (C region) of A bands in striated muscle. MYBPC3, the cardiac isoform, is expressed exclussively in heart muscle. Regulatory phosphorylation of the cardiac isoform in vivo by cAMP-dependent protein kinase (PKA) upon adrenergic stimulation may be linked to modulation of cardiac contraction. Mutations in MYBPC3 are one cause of familial hypertrophic cardiomyopathy. |
Specification |
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|---|---|
| Aliases | Myosin-binding protein C, cardiac-type, Cardiac MyBP-C, C-protein, cardiac muscle isoform, MYBPC3 |
| Entrez GeneID | 4607 |
| Swissprot | Q14896 |
| WB Predicted band size | 140.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This MYBPC3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-218 amino acids from the N-terminal region of human MYBPC3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
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|---|---|
| WB | 1/2000 |
| IHC | 1/100-1/500 |
Product Image
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Anti-MYBPC3 Antibody (N-term) at 1:2000 dilution + rat heart lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size :141kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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P33721 staining MYBPC3 in human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
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