Rabbit Polyclonal Antibody to PHF5A
货号:
P33737
别名:
PHD finger-like domain-containing protein 5A, PHD finger-like domain protein 5A, Splicing factor 3B-associated 14 kDa protein, SF3b14b, PHF5A
应用:
WB,FCM
反应种属:
Human
抗体类型:
Primary antibody
Swissprot:
Q7RTV0
规格:
目录价
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Description |
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This gene encodes a subunit of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. The protein encoded by this gene contains a PHD-finger-like domain that is flanked by highly basic N- and C-termini. This protein belongs to the PHD-finger superfamily and may act as a chromatin-associated protein. This gene has several pseudogenes on different chromosomes. |
Specification |
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Aliases | PHD finger-like domain-containing protein 5A, PHD finger-like domain protein 5A, Splicing factor 3B-associated 14 kDa protein, SF3b14b, PHF5A |
Entrez GeneID | 84844 |
Swissprot | Q7RTV0 |
WB Predicted band size | 12.4kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human |
Immunogen | This PHF5A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-110 amino acids from the C-terminal region of human PHF5A. |
Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
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WB | 1/1000 |
FCM | 1/10-1/50 |
Product Image
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All lanes : Anti-PHF5A Antibody (C-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: HL-60 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 12 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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PHF5A Antibody (C-term) (Cat. #P33737) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.