Rabbit Polyclonal Antibody to RASIP1
货号：
P33860
别名：
Ras-interacting protein 1, Rain, RASIP1
应用：
WB,IHC,FCM
反应种属：
Human, Mouse
抗体类型：
Primary antibody
Swissprot：
Q5U651
规格：
目录价
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Description |
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RASIP1 may act as effector for Golgi-bound HRAS and other Ras-like proteins. May promote HRAS-mediated transformation. |
Specification |
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|---|---|
| Aliases | Ras-interacting protein 1, Rain, RASIP1 |
| Entrez GeneID | 54922 |
| Swissprot | Q5U651 |
| WB Predicted band size | 103.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This RASIP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 651-678 amino acids from the Central region of human RASIP1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
Application |
|
|---|---|
| WB | 1/1000-1/2000 |
| IHC | 1/100-1/500 |
| FCM | 1/25 |
Product Image
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All lanes : Anti-RASIP1 Antibody (Center) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: HUVEC whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 103 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Western blot analysis of RASIP1 Antibody (Center) (Cat. #P33860) in K562 cell line lysates (35ug/lane). RASIP1 (arrow) was detected using the purified Pab.
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Overlay histogram showing K562 cells stained with P33860 (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33860, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Formalin-fixed and paraffin-embedded human brain tissue reacted with RASIP1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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